Presents a reference on various aspects of the isolation and cultivation of marine and freshwater algae, including seaweeds. This book covers history, media preparation, isolation and purification, mass culturing techniques, cell counting and growth measurement techniques, and reviews on topics and applications of algal culture techniques. Contributors vii Preface ix Historical Review of Algal Culturing Techniques 1(12) Hans R. Preisig Robert A. Andersen Freshwater Culture Media 13(8) Makoto M. Watanabe Marine Culture Media 21(14) Paul J. Harrison John A. Berges Trace Metal Ion Buffers and Their Use in Culture Studies 35(30) William G. Sunda Neil M. Price Francois M. M. Morel Sterilization and Sterile Technique 65(18) Masanobu Kawachi Mary-Helene Noel Traditional Microalgae Isolation Techniques 83(18) Robert A. Andersen Masanobu Kawachi Automated Isolation Techniques for Microalgae 101(16) Michael Sieracki Nicole Poulton Nicholas Crosbie Purification Methods for Microalgae 117(16) Robert R. L. Guillard Isolation and Purification Techniques for Macroalgae 133(12) Hiroshi Kawai Taizo Motomura Kazuo Okuda Perpetual Maintenance of Actively Metabolizing Microalgal Cultures 145(12) Maike Lorenz Thomas Friedl John G. Day Long-Term Macroalgal Culture Maintenance 157(8) John A. West Cryopreservation Methods for Maintaining Microalgal Cultures 165(24) John G. Day Jerry J. Brand Photobioreactors and Fermentors: The Light and Dark Sides of Growing Algae 189(16) Paul W. Behrens Culturing Microalgae in Outdoor Ponds 205(14) Michael A. Borowitzka Mariculture of Seaweeds 219(20) Dinabandhu Sahoo Charles Yarish Counting Cells in Cultures with the Light Microscope 239(14) Robert R. L. Guillard Michael S. Sieracki Phytoplankton Cell Counting by Flow Cytometry 253(16) Dominique Marie Nathalie Simon Daniel Vaulot Measuring Growth Rates in Microalgal Cultures 269(18) A. Michelle Wood R. C. Everroad L. M. Wingard Using Cultures to Investigate the Physiological Ecology of Microalgae 287(40) Hugh L. MacIntyre John J. Cullen Analysis of Algal Pigments by High-Performance Liquid Chromatography 327(20) Robert R. Bidigare Laurie Van Heukelem Charles C. Trees Endogenous Rhythms and Daylength Effects in Macroalgal Development 347(18) Klaus Luning Viral Contamination of Algal Cultures 365(24) Janice Lawrence Control of Sexual Reproduction in Algae in Culture 389(10) Annette W. Coleman Thomas Proschold Microalgal Life Cycles: Encystment and Excystment 399(20) Susan Blackburn Naomi Parker Cultures as a Means of Protecting Biological Resources: Ex Situ Conservation of Threatened Algal Species 419(10) Makoto M. Watanabe Appendix A Recipes for Freshwater and Seawater Media 429(110) R. A. Andersen J. A. Berges P. J. Harrison M. M. Watanabe Glossary 539(6) Name Index 545(20) Subject Index 565