Fifteen years ago the field of oxygen free radicals was just beginning to launch into a new area of importance in pathology. Since then, oxygen free radicals have been implicated in a number of disease processes, including atherosclerosis and chronic inflammation. Oxygen free radicals have been implicated in a number of disease processes. This book provides a comprehensive guide for methodology in the analysis of biological samples. It is designed to help the clinical scientist evaluate the best method for their particular problem. CHROMATOGRAPHIC PROCEDURES.
Lipid Peroxidation Determination by HPLC (R. Bevan).
Measurement of Oxidized Bases in DNA and Biological Fluids by Gas Chromatography Coupled to Mass Spectrometry (T. Douki, et al.).
The Measurement of Protein Oxidation by HPLC (H. Griffiths, et al.).
MEASUREMENT OF 8 OXO DEOXYGUANOSINE.
Measurement of 8 Oxo 2 deoxyguanosine in Cellular DNA by High Performance Liquid Chromatography Electrochemical Detection (M. Evans).
Immunochemical Detection of 8 Oxodeoxyguanosine in DNA (M. Cooke & K. Herbert).
Urinary Measurement of 8 OxodG (8 Oxo 2 deoxyguanosine) (H. Poulsen, et al.).
CELLULAR BASED METHODS.
Measurement of Oxidative DNA Damage Using the Comet Assay (A. Collins).
Measuring Oxidative DNA Damage by Alkaline Elution (M. Pflaum & B. Epe).
MOLECULAR BASED ASSAYS.
A ¯32P Postlabelling Protocol to Measure Oxidative DNA Damage (G. Jones & M. Weinfeld).
Mapping Reactive Oxygen Induced DNA Damage at Nucleotide Resolution (H. Rodriguez & S. Akman).
ANTIOXIDANT ACTIVITY.
Measurements of Plasma Antioxidant Activity (S. Maxwell).
Index.