Edited by an interdisciplinary team of scientists at one of the leading centers for microsystem research, this second edition retains the proven structure of its predecessor, enlarged by around 10%. Focusing on analytical applications mainly in life sciences, this is an invaluable companion for fast and automated analytical procedures, providing a concise practical approach to microtechnology. This expanded second edition of the concise practical approach to microsystem technology focuses on analytical applications in life sciences at a non-specialist level, clearly illuminating the principles of the design and manufacture of lab-on-a-chip devices. Preface. 1. Introduction (Pieter Telleman).  1.1. Learning from the Experiences of Microelectronics. 1.2. The Advantages of Miniaturizing Systems for Chemical Analysis. 1.3. From Concept to µTAS. 1.4. References. 2. Clean Rooms (Daria Petersen and Pieter Telleman). 3. Microfluidics Theoretical Aspects (Jörg P. Kutter and Henning Klank). 3.1. Fluids and Flows. 3.2. Transport Processes. 3.3.  System Design. 3.4. An Application: Biological Fluids,. 3.5. References. 4. Microfluidics Components (Jörg P. Kutter, Klaus Bo Mogensen, Henning Klank, and Oliver Geschke). 4.1. Valves and Pumps. 4.2. Injecting, Dosing, and Metering. 4.3. Temperature Measurement in Microfluidic Systems. 4.4. Optical Sensors. 4.5. Electrochemical Sensors. 4.6. References. 5. Simulations in Microfluidics (Goran Goranovic and Henrick Bruus). 5.1. Physical Aspects and Design. 5.2. Choosing Software and Hardware. 5.3. Important Numerical Settings. 5.4. Errors and Uncertainties. 5.5. Interpretation and Evaluation of Simulations. 5.6. Example Simulations. 5.7. References. 6. Silicon and Cleanroom Processing (Anders Michael Jorgensen and Klaus Bo Mogensen). 6.1. Substrate Fabrication. 6.2. Optical Lithography. 6.3. Deposition. 6.4. Etching. 6.5. Dry Etching. 6.6. Heat Treatment. 6.7. References. 7. Glass Micromachining (Daria Petersen, Klaus Bo Mogensen, and Henning Klank). 7.1. Wet Chemical Etching. 7.2. Reactive Ion Etching (RIE) of Glass. 7.3. Laser Patterning. 7.4. Powder Blasting. 7.5. Glass Bonding. 7.6. A Microfabrication Example. 7.7. References. 8. Polymer Micromachining (Oliver Geschke, Henning Klank, and Klaus Bo Mogensen. 8.1. Hot Embossing. 8.2. Injection Molding. 8.3. Casting. 8.4. Laser Micromachining. 8.5. Milling. 8.6. X ray and Ultraviolet Polymer Lithography. 8.7. Sealing of Polymer Microstructures. 8.8. Adding Functionalities. 8.9 Examples of Polymer Microstructures. 8.10. References. 9. Packaging of microfluidic Systems (Gerardo Perozziello). 9.1. Levels of packaging. 9.2. Factors influencing the packaging design and reliability. 9.3. Materials. 9.4. Interconnections. 9.5. References. 10. Determination of Topography (Henning Klank). 10.1. Topography General Discussion. 10.2. Importance and Relevance of Topography. 10.3. Topographical Determination Considerations. 10.4. Topographical Characterization Methods and Instruments. 10.5. Topographical Examination of a Typical Sample. 10.8 References. 11. Analytical Chemistry on Microsystems (Jörg Kutter and Oliver Geschke). 11.1. Sensors and Sensor Systems. 11.2. Biosensors. 11.3. Flow Injection Analysis. 11.4. Separation Techniques. 11.5. Other Analytical Techniques. 11.6. References. Subject Index.